Prokaryotes Community Analysis of Rhizosphere of rice plants treated ... - SRA

SRX23086175: Prokaryotes Community Analysis of Rhizosphere of rice plants treated with MiZax3, milky stage
1 ILLUMINA (Illumina NovaSeq 6000) run: 329,047 spots, 164.5M bases, 60.3Mb downloads

Design: Prokaryotes community assembly was analyzed by using the V4 hyper-variable region of the 16S rDNA using 515F-805R primers pair (Parada et al. 2016; Apprill et al. 2015) linked with the Illumina adapters overhang. Amplification on organellar rRNA was prevented by peptide nucleic acids (PNAs) clamping using universal pPNA and mPNA clamps for plastidial and mitochondrial sequences following the manufacturers protocol (PNA Bio Inc, Newbury Park, CA).

Submitted by: University of Torino

Study: Zaxinone and its mimics modulate the rice root microbiota

PRJNA1060774 • SRP481150 • All experiments • All runs

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Rice plants (cv. Nipponbare) grown on native paddy soil and bulk soil were treated with Zaxinone, MiZax3 and MiZax5 during two different phenological phases (tillering and milky stages) to asses the bacterial and fungal communities recruited by the rhizosphere, root (endosphere), and soil using 16S rRNA ITS2 amplicon sequencing, respectively.

Sample: Rhizosphere of rice plants treated with MiZax3, milky stage

SAMN39244700 • SRS20043543 • All experiments • All runs

Organism: rhizosphere metagenome

Library:

Name: 92_16S

Instrument: Illumina NovaSeq 6000

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 329,047 spots, 164.5M bases, 60.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR27411350	329,047	164.5M	60.3Mb	2024-12-28

ID:: 31179508

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